It involves stimulation of the female partner to produce more number of eggs at a time. When the majority of these eggs mature, ovum-pick-up (OPU) is scheduled. OPU is a daycare procedure which is done under sedation. The eggs retrieved are cultured for 3-4 hours before proceeding IVF with washed sperms. The calculated number of sperms are released in the vicinity of the eggs and fertilization occurs naturally with one sperm entering the egg to form an embryo.